Effect of PGR pulse treatments and ethylene precursor and inhibitors on Scots pine micropropagation

Background Conifer clonal forestry as a form of plantation forestry has great potential advantages. However, vegetative propagation of conifers, especially that of mature trees, is quite problematic. These species also are most recalcitrant objects for cultivation in vitro. For this reason the development of an effective system for clonal micropropagation of some conifers is still an important aim. Scots pine (Pinus sylvestris L.) is one of the most widespread conifers in the world and in Russia. This important species has high economic value. Among Pinus species, Scots pine is especially difficult to deal with in culture [1]. We investigated influence of pulse treatment with BA, GA and auxins on clonal micropropagation of Scots pine. In addition effects of ethylene precursor and inhibitors were analyzed.